BCJ 3601 Unit IV Case Study
This case study assignment explores euthanasia. Using the CSU Online Library and additional scholarly resources, research euthanasia in your state, and address the items below.
Your case study will be at least two pages in length, not counting the title and reference pages. It should present an insightful and thorough analysis with strong arguments and evidence.
While you may use your textbook to complete this assignment, you are required to use at least two outside resources, one of which must be from the CSU Online Library. All resources used, including the textbook, must be referenced; paraphrased and quoted material must have accompanying citations. Your case study, including all references, will be formatted in APA style.
This is from the CSU ONLINE LIBRARY
Findings on Lung Injury Detailed by Investigators at Duke University (Euthanasia- and Lavage-mediated Effects on Bronchoalveolar Measures of Lung Injury and Inflammation)
(Aug. 20, 2018): p2733.
Copyright: COPYRIGHT 2018 NewsRX LLChttp://www.newsrx.com/newsletters/Respiratory-Therapeutics-Week.html
2018 AUG 20 (NewsRx) — By a News Reporter-Staff News Editor at Respiratory Therapeutics Week — Investigators publish new report on Lung Diseases and Conditions – Lung Injury. According to news originating from Durham, North Carolina, by NewsRx correspondents, research stated, “Accurate and reproducible assessments of experimental lung injury and inflammation are critical for basic and translational research. In particular, investigators use various methods for BAL and euthanasia; however, the impact of these methods on assessments of injury and inflammation is unknown.”
Our news journalists obtained a quote from the research from Duke University, “To define potential effects, we compared methods of lavage and euthanasia in uninjured mice and after a mild lung injury model (ozone). C57BL/6J male mice (8-10 weeks old) underwent BAL after euthanasia with ketamine/xylazine, carbon dioxide (CO2), or isoflurane. BAL methods included 800 mu l of isotonic solution instilled and withdrawn three times, and one or three passive fills and drainage to 20 cm H2O. Parallel experiments were performed 24 hours after 3 hours of ozone (O-3) exposure at 2 ppm. BAL total cell counts/differentials and total protein/albumin were determined. Lung histology was evaluated for lung inflammation or injury. BAL cells were cultured and stimulated with PBS, PMA, or LPS for 4 hours and supernatants were evaluated for cytokine content. In uninjured mice, we observed differences due to the lavage and euthanasia methods used. The lavage method increased total cells and total protein/albumin in uninjured and O-3 -exposed mice, with the 800-mu l instillation having the highest values. Isoflurane increased total BAL cells, whereas CO(2 )euthanasia increased the total protein/albumin levels in uninjured mice. These effects limited our ability to detect differences in BAL injury measures after O-3 exposure.”
According to the news editors, the research concluded: “The method used for lavage and euthanasia affects measures of lung inflammation/injury and should be considered a variable in model assessments.”
For more information on this research see: Euthanasia- and Lavage-mediated Effects on Bronchoalveolar Measures of Lung Injury and Inflammation. American Journal of Respiratory Cell and Molecular Biology, 2018;59(2):257-266. American Journal of Respiratory Cell and Molecular Biology can be contacted at: Amer Thoracic Soc, 25 Broadway, 18 Fl, New York, NY 10004, USA.
The news correspondents report that additional information may be obtained from R.M. Tighe, Duke University, Dept. of Med, Durham, NC 27710, United States. Additional authors for this research include A. Birukova, M.J. Yaeger, S.W. Reece and K.M. Gowdy.
The direct object identifier (DOI) for that additional information is: https://doi-org.libraryresources.columbiasouthern.edu/10.1165/rcmb.2017-0357OC. This DOI is a link to an online electronic document that is either free or for purchase, and can be your direct source for a journal article and its citation.
Keywords for this news article include: Durham, North Carolina, United States, North and Central America, Respiratory Tract Diseases and Conditions, Lung Diseases and Conditions, Health and Medicine, Terminal Care, Inflammation, Lung Injury, Euthanasia, Duke University.
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2018, NewsRx LLC
The citation for this news report is: NewsRx. Findings on Lung Injury Detailed by Investigators at Duke University (Euthanasia- and Lavage-mediated Effects on Bronchoalveolar Measures of Lung Injury and Inflammation). Respiratory Therapeutics Week. August 20, 2018; p 2733.
Source Citation (MLA 8th Edition)“Findings on Lung Injury Detailed by Investigators at Duke University (Euthanasia- and Lavage-mediated Effects on Bronchoalveolar Measures of Lung Injury and Inflammation).” Respiratory Therapeutics Week, 20 Aug. 2018, p. 2733. Academic OneFile, http://link.galegroup.com/apps/doc/A550725268/AONE…. Accessed 24 Apr. 2019.